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pSpCas9_human_ADAR2_ccB
(Plasmid #158113)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158113 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 158113-D50 50 μg of DNA in Tris buffer $495
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DNA 158113-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pSpCas9(BB)-2A-Puro PX459
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 9175
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ADAR2
  • gRNA/shRNA sequence
    CACC G TATGACTTCCTCTCCGAGAG
  • Species
    H. sapiens (human)
  • GenBank ID
    104
  • Entrez Gene
    ADARB1 (a.k.a. ADAR2, DRABA2, DRADA2, NEDHYMS, RED1)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.07.12.499727 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSpCas9_human_ADAR2_ccB was a gift from Emanuela Felley-Bosco (Addgene plasmid # 158113 ; http://n2t.net/addgene:158113 ; RRID:Addgene_158113)

  • For your References section:

    Heterogeneous RNA editing and influence of ADAR2 on mesothelioma chemoresistance and the tumor microenvironment. Hariharan A, Qi W, Rehrauer H, Wu L, Ronner M, Wipplinger M, Kresoja-Rakic J, Sun S, Oton-Gonzalez L, Sculco M, Serre-Beinier V, Meiller C, Blanquart C, Fonteneau JF, Vrugt B, Ruschoff JH, Opitz I, Jean D, de Perrot M, Felley-Bosco E. Mol Oncol. 2022 Dec;16(22):3949-3974. doi: 10.1002/1878-0261.13322. Epub 2022 Oct 31. 10.1002/1878-0261.13322 PubMed 36221913
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