PurposeHigh Copy number plasmid containing Ple341 (PCP2 MiniPromoter) insert
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||158392||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 3518
Growth in Bacteria
Copy numberHigh Copy
Alt namePCP2 MiniPromoter
SpeciesH. sapiens (human)
Insert Size (bp)800
Entrez GenePCP2 (a.k.a. GPSM4)
- Promoter Ple341
- Cloning method Restriction Enzyme
- 5′ cloning site FseI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer M13-fwd
- 3′ sequencing primer M13-rev (Common Sequencing Primers)
Plasmid commercially produced by Genscript . Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid, including the MiniPromoter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEMS2267 was a gift from Elizabeth Simpson (Addgene plasmid # 158392 ; http://n2t.net/addgene:158392 ; RRID:Addgene_158392)
For your References section:Human MiniPromoters for ocular-rAAV expression in ON bipolar, cone, corneal, endothelial, Muller glial, and PAX6 cells. Korecki AJ, Cueva-Vargas JL, Fornes O, Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Zhou M, Wasserman WW, Di Polo A, Simpson EM. Gene Ther. 2021 Feb 2. pii: 10.1038/s41434-021-00227-z. doi: 10.1038/s41434-021-00227-z. 10.1038/s41434-021-00227-z PubMed 33531684