pB6-Id1-IRES-loxP-NeoR-loxP-DTA-TK
(Plasmid #158616)

• Purpose: B6J Id1-LSL-DTA allele targeting vector, low copy number
• Depositing Lab: Mario Capecchi
• Publication: Miscellaneous gene targeting vector plasmids (unpublished)

Backbone

• Vector backbone: pWS-TK6
• Backbone manufacturer: Sen Wu, Capecchi laboratory
• Vector type: Mammalian Expression, Mouse Targeting, Cre/Lox
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Id1
• Species: M. musculus (mouse)
• Mutation: Insertion of IRES-loxP-NeoR-loxP-DTA after the stop codon, 3 bases into the 3' untranslated region (per Mona Khan, Mombaerts laboratory)

Resource Information

• Supplemental Documents:
  • Annotated Depositor's Sequence
• A portion of this plasmid was derived from a plasmid made by: IRES-loxP-NeoR-loxP-DTA is from plasmid Gdf7-IRES-loxNeolox-DTA from Thomas Jessell.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note there are several ambiguous bases in Addgene's NGS sequence. Depositor states these are repetitive or GC-rich sequences. These regions will not affect plasmid function.

Homology arms subcloned by recombineering from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.

Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.