pSWD56
(Plasmid #158619)

• Purpose: Long Fibronectin FRET Standard
• Depositing Lab: Seth Childers
• Publication: Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. doi: 10.1021/acssensors.0c00008. Epub 2020 Jun 16.

Backbone

• Vector backbone: pXCFPC-6
• Backbone size w/o insert (bp): 4828
• Total vector size (bp): 6731
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Long FRET Standard
• Alt name: mClover3-Fibronectin Units 7-8-mRuby3
• Species: Synthetic
• Insert Size (bp): 1971
• Promoter: Xylose

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CCCACATGTTAGCGCTACCAAGTGC
• 3′ sequencing primer: GCCAGGGTTTTCCCAGTCACGA

Resource Information

• Supplemental Documents:
  • pSWD56.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Vector from Martin Thanbichler PM ID 17959646 "A comprehensive set of plasmids for vanillate- and xylose-inducible gene expression in Caulobacter crescentus".