pEN_TT 3xFLAG-MAVS-Ala148,Ala271
(Plasmid #158633)

• Purpose: Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant
• Depositing Lab: Kevin Janes
• Publication: Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13. doi: 10.1016/j.cels.2021.02.004. Epub 2021 Mar 18.

Backbone

• Vector backbone: pEN_TTmiR2c_3xFLAG
• Backbone manufacturer: Addgene #83274
• Vector type: Gateway entry vector

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MAVS
• Alt name: IPS-1
• Alt name: VISA
• Species: H. sapiens (human)
• Mutation: Q148A/Q271A
• Entrez Gene: MAVS (a.k.a. CARDIF, IPS-1, IPS1, VISA)
• Tag / Fusion Protein:
  • 3XFLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: MfeI (not destroyed)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid differs from the MAVS reference sequence at 3 position (Q93E, Q198K, and S409F). This plasmid has an additional Q148A and Q271A mutation. Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) two times on the pEN_TT 3XFLAG-MAVS plasmid: one mutagenesis to yield pEN_TT 3XFLAG-MAVS-Q148A and a second mutagenesis to yield the double mutant.