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LifeAct-14 (LA-14)
(Plasmid #158750)


Item Catalog # Description Quantity Price (USD)
Plasmid 158750 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pMuLE Dest BlastR
  • Backbone manufacturer
    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi: 10.1038/s41467-018-05367-2.
  • Backbone size w/o insert (bp) 8467
  • Total vector size (bp) 8509
  • Modifications to backbone
    Inserted LA-14 sequence in the Addgene plasmid # 84383
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Can grow at 37C also
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
  • Alt name
    Shorter variant of actin binding LifeAct (amino acid 1-14)
  • Insert Size (bp)
  • Mutation
    Deletion mutation for last three amino acids from LifeAct sequence
  • Promoter CMV immediate early
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Performed site-directed mutagenesis (SDM) to introduced deletion mutations for the terminal 3 amino acids in LifeAct sequence cloned in Addgene plasmid # 84383

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LifeAct-14 (LA-14) was a gift from Minhajuddin Sirajuddin (Addgene plasmid # 158750 ; ; RRID:Addgene_158750)
  • For your References section:

    Structural insights into actin filament recognition by commonly used cellular actin markers. Kumari A, Kesarwani S, Javoor MG, Vinothkumar KR, Sirajuddin M. EMBO J. 2020 Jun 22:e104006. doi: 10.15252/embj.2019104006. 10.15252/embj.2019104006 PubMed 32567727