pSWD95D293A
(Plasmid #159099)

• Purpose: CckA FRET Sensor with DITE motif mutant
• Depositing Lab: Seth Childers
• Publication: Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. doi: 10.1021/acssensors.0c00008. Epub 2020 Jun 16.

Backbone

• Vector backbone: pXCFPC-6
• Backbone size w/o insert (bp): 4828
• Total vector size (bp): 8389
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3
• Alt name: CckA FRET Sensor
• Species: Caulobacter crescentus
• Insert Size (bp): 3558
• Mutation: CckA D293 mutated to A293 and V199I on mRuby3 (Please see depositor comments)
• GenBank ID: AF133718.1
• Promoter: Xylose

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CCCACATGTTAGCGCTACCAAGTGC
• 3′ sequencing primer: GCCAGGGTTTTCCCAGTCACGA

Resource Information

• Supplemental Documents:
  • pSWD95D293A.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Vector from Martin Thanbichler PM ID 17959646 "A comprehensive set of plasmids for vanillate- and xylose-inducible gene expression in Caulobacter crescentus".

Addgene NGS result had V199I on mRuby3. Depositor confirmed that this mutation does not affect the plasmid function.