|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15914||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneLTRi (with neomycin R)
- Backbone size w/o insert (bp) 11100
Vector typeMammalian Expression, Synthetic Biology
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsStratagene SURE Very low copy: TLD recommends Megapreps
Copy numberLow Copy
Insert Size (bp)800
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
We engineered a tunable genetic switch called LTRi, which stands for Lac-Tet-RNAi. Gene expression is turned on by adding an inducer, isopropyl-β-thiogalactopyranoside (IPTG). LTRi-EGFP allows scientists to tune the level of EGFP. See article for details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LTRi EGFP was a gift from James Collins (Addgene plasmid # 15914)
For your References section:A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells. Deans TL, Cantor CR, Collins JJ. Cell. 2007 Jul 27. 130(2):363-72. 10.1016/j.cell.2007.05.045 PubMed 17662949