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TOPO mMecp2-NLucTom
(Plasmid #159282)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159282 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 159282-D50 50 μg of DNA in Tris buffer $495
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DNA 159282-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    TOPO
  • Backbone size w/o insert (bp) 3424
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mecp2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3157
  • Mutation
    Nluciferase-TdTomato fusion
  • Entrez Gene
    Mecp2 (a.k.a. 1500041B07Rik, D630021H01Rik, Mbd5, WBP10)
  • Tag / Fusion Protein
    • Nluciferase-P2A-TdTomato (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Both homology arms are from a castaneus background. This plasmid was used to target the C-terminus of mouse Mecp2 to fuse it in frame with a NanoLuciferase reporter and an additional TdTomato. There is a P2A signal between Nluc and Tomato leading to Tomato being translated as an independent protein.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TOPO mMecp2-NLucTom was a gift from Joost Gribnau (Addgene plasmid # 159282 ; http://n2t.net/addgene:159282 ; RRID:Addgene_159282)

  • For your References section:

    Genetic and epigenetic determinants of reactivation of Mecp2 and the inactive X chromosome in neural stem cells. Mira-Bontenbal H, Tan B, Gontan C, Goossens S, Boers RG, Boers JB, Dupont C, van Royen ME, IJcken WFJ, French P, Bedalov A, Gribnau J. Stem Cell Reports. 2022 Jan 29. pii: S2213-6711(22)00052-2. doi: 10.1016/j.stemcr.2022.01.008. 10.1016/j.stemcr.2022.01.008 PubMed 35148843
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