Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15930||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepGEM-T Easy
- Backbone size w/o insert (bp) 3015
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)1634
Entrez GeneGnao1 (a.k.a. AW050213, Galphao, Gnao, alphaO)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
ApaI digestion and SP6 RNA polymerase for antisense probe
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pISH-Galpha-o was a gift from Peter Mombaerts (Addgene plasmid # 15930 ; http://n2t.net/addgene:15930 ; RRID:Addgene_15930)
For your References section:Multiple new and isolated families within the mouse superfamily of V1r vomeronasal receptors. Rodriguez I, Del Punta K, Rothman A, Ishii T, Mombaerts P. Nat Neurosci. 2002 Feb . 5(2):134-40. 10.1038/nn795 PubMed 11802169