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pET15-EF1n3_mFAP2b
(Plasmid #159472)

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  • Purpose
    Encodes for EF1n3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
  • Depositing Lab
    David Baker
  • Depositing Organization
    University of Washington
    Located in United States of America
  • Publication
    Baker Lab mFAP plasmids (unpublished) ( How to cite )
  • Sequence Information

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159472 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET15
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EF1n3_mFAP2b
  • Species
    E. coli
  • Tag / Fusion Protein
    • 6xHis-thrombin-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15-EF1n3_mFAP2b was a gift from David Baker (Addgene plasmid # 159472 ; http://n2t.net/addgene:159472 ; RRID:Addgene_159472)
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