pOD_009
(Plasmid
#159520)
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Purpose(Empty Backbone) Rifampicin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159520 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepDEL
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Backbone manufacturerIlya B. Tikh and James C. Samuelson1
- Backbone size (bp) 4282
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Modifications to backboneReplacement of KanR resistance cassette by Rifampicin resistance cassette. Rifampicin cassette was amplified from TT23430 from Roth lab ( http://rothlab.ucdavis.edu/cgi-bin/textQuery.cgi )
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Rifampicin
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Copy numberUnknown
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
- The destination strain should be recA+ and not be able to maintain R6K plasmid
- Zeocin is used to select colonies with the integrated vector.
- The rhaBAD and lac promoters can be induced with 0.2% rhamnose and 0.5mM IPTG
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOD_009 was a gift from Elisabeth Raleigh (Addgene plasmid # 159520 ; http://n2t.net/addgene:159520 ; RRID:Addgene_159520) -
For your References section:
Reassembling a cannon in the DNA defense arsenal: Genetics of StySA, a BREX phage exclusion system in Salmonella lab strains. Zaworski J, Dagva O, Brandt J, Baum C, Ettwiller L, Fomenkov A, Raleigh EA. PLoS Genet. 2022 Apr 4;18(4):e1009943. doi: 10.1371/journal.pgen.1009943. eCollection 2022 Apr. 10.1371/journal.pgen.1009943 PubMed 35377874
Map uploaded by the depositor.
