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(Plasmid #159526)


Item Catalog # Description Quantity Price (USD)
Plasmid 159526 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pAAV-Ef1a-mCherry (Addgene 114470)
  • Backbone manufacturer
    Karl Deisseroth and Stratagene
  • Backbone size w/o insert (bp) 4948
  • Total vector size (bp) 5659
  • Modifications to backbone
    Replaced EF1a with LAP1_2
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Insert Size (bp)
  • Promoter LAP1_2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GCA CGC GTA TCT CCG GAA AGA GGA AAT TGA
  • 3′ sequencing primer GCG GAT CCG AGC TCC CTC TTC CTC GCC GCG GAC TGG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-LAP1_2-mCherry was a gift from Esteban Engel (Addgene plasmid # 159526 ; ; RRID:Addgene_159526)
  • For your References section:

    Small Alphaherpesvirus Latency-Associated Promoters Drive Efficient and Long-Term Transgene Expression in the CNS. Maturana CJ, Verpeut JL, Pisano TJ, Dhanerawala ZM, Esteves A, Enquist LW, Engel EA. Mol Ther Methods Clin Dev. 2020 Apr 14;17:843-857. doi: 10.1016/j.omtm.2020.04.004. eCollection 2020 Jun 12. 10.1016/j.omtm.2020.04.004 PubMed 32368565