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pM50-ISH
(Plasmid #15955)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15955 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    M12
  • Alt name
    MOR103-16
  • Alt name
    olfr6
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1721
  • GenBank ID
    AF321236
  • Entrez Gene
    Olfr6 (a.k.a. M50, MOR103-16)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer M13 reverse
  • 3′ sequencing primer M13 forward
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

to make RNA probe, cut with NotI. Then do in vitro transcription with T7RNA polymerase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pM50-ISH was a gift from Peter Mombaerts (Addgene plasmid # 15955 ; http://n2t.net/addgene:15955 ; RRID:Addgene_15955)
  • For your References section:

    Local and cis Effects of the H Element on Expression of Odorant Receptor Genes in Mouse. Fuss SH, Omura M, Mombaerts P. Cell. 2007 Jul 27. 130(2):373-84. 10.1016/j.cell.2007.06.023 PubMed 17662950