pAAV-CAG-NIR-GECO2
(Plasmid #159603)

• Purpose: AAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2
• Depositing Lab: Robert Campbell
• Publication: Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965. doi: 10.1371/journal.pbio.3000965. eCollection 2020 Nov.

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Backbone

• Vector backbone: pAAV-CAG
• Backbone size w/o insert (bp): 4732
• Total vector size (bp): 6220
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NIR-GECO2
• Species: Synthetic
• Insert Size (bp): 1488
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CAG-Fw
• 3′ sequencing primer: WPRE-Rv

Resource Information

• Supplemental Documents:
  • pAAV-CAG-NIR-GECO2.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.04.08.032433v2 for bioRxiv preprint.