pAAV-CAG-NIR-GECO2
(Plasmid
#159603)
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PurposeAAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159603 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV-CAG
- Backbone size w/o insert (bp) 4732
- Total vector size (bp) 6220
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNIR-GECO2
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SpeciesSynthetic
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Insert Size (bp)1488
- Promoter CAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CAG-Fw
- 3′ sequencing primer WPRE-Rv (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CAG-NIR-GECO2 was a gift from Robert Campbell (Addgene plasmid # 159603 ; http://n2t.net/addgene:159603 ; RRID:Addgene_159603)