R989-X79-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L
(Plasmid
#159692)
-
PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159692 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepDest-636
-
Vector typeInsect Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameHis6-MBP-tev-G-Hs.KRAS4b(1-188) M188L
-
SpeciesH. sapiens (human)
-
MutationM188L
-
Entrez GeneKRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
-
Tag
/ Fusion Protein
- His6-MBP-tev (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
R989-X79-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L was a gift from Dominic Esposito (Addgene plasmid # 159692 ; http://n2t.net/addgene:159692 ; RRID:Addgene_159692) -
For your References section:
Production of authentic geranylgeranylated KRAS4b using an engineered baculovirus system. Procter L, Grose C, Esposito D. Protein Expr Purif. 2018 Nov;151:99-105. doi: 10.1016/j.pep.2018.06.012. Epub 2018 Jun 22. 10.1016/j.pep.2018.06.012 PubMed 29936133
