pAM1479a3
(Plasmid #159725)

• Purpose: Express MYITV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg
• Depositing Lab: Alexander Varshavsky
• Publication: Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116(32):15914-15923. doi: 10.1073/pnas.1908304116. Epub 2019 Jul 23.

Backbone

• Vector backbone: pGBKCg
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mDHFR
• Species: M. musculus (mouse)
• Mutation: remove start and stop codon of Dhfr
• Entrez Gene: Dhfr (a.k.a. 8430436I03Rik)
• Promoter: ADH1
• Tags / Fusion Proteins:
  • MYITV-GGGGGG (N terminal on insert)
  • Gal4DBD (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pAM1479-a3.gbk
• A portion of this plasmid was derived from a plasmid made by: Lab collection

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For 3' clonging site, a XhoI site that is present naturally in the sequence of a segment encoding the Gal4 DBD.