pDblet-swi1+
(Plasmid #15981)

• Depositing Lab: Eishi Noguchi
• Publication: Noguchi et al Mol Cell Biol. 2003 Nov . 23(21):7861-74.

Backbone

• Vector backbone: pDblet
• Backbone size w/o insert (bp): 6200
• Vector type: Yeast Expression
• Selectable markers: URA4 (S. pombe equivalent to URA3)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Swi1
• Species: S. pombe (fission yeast)
• Insert Size (bp): 3678
• Entrez Gene: swi1 (a.k.a. SPBC216.06c)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: M13R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry