|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||160066||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Copy numberHigh Copy
SpeciesR. norvegicus (rat)
- Promoter mouse U6 promoter
- Cloning method Restriction Enzyme
- 5′ cloning site BbsI (unknown if destroyed)
- 3′ cloning site Xba (unknown if destroyed)
A portion of this plasmid was derived from a plasmid made byInsert was originally cloned in Angel de Blas's lab.
Terms and Licenses
- Not Available to Industry
Targets collybistin SH3+ mRNAs (target is located in the protein coding region of SH3+. Nucleotides 517-537 of AJ250425 [CB1SH3+]. Targets collybistin isoforms with SH3 domain). There is also a control mutated shRNA for this isoform (Addgene plasmid #160067).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CBSH3+ shRNA-1 was a gift from Angel de Blas (Addgene plasmid # 160066 ; http://n2t.net/addgene:160066 ; RRID:Addgene_160066)