pGFAP::MyrGFP_v2
(Plasmid #160277)

• Purpose: Expresses GFP from the Gfa2 promoter. This plasmid is a new version of Plasmid 22672 and includes a stop codon in the myrEGFP insert.
• Depositing Lab: Robert Benezra
• Depositing Organization: Memorial Sloan-Kettering Cancer Center
  Located in the United States of America
• Publication: Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Backbone

• Vector backbone: Gfa2-cLac
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: myrGFP
• Insert Size (bp): 700
• Promoter: Gfa2

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Michael Brenner [mailto:]

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a new version of Plasmid 22672: pGFAP::MyrGFP. A stop codon at the 3' end of myrEGFP prevents translation of the mouse Protamine 1 coding sequence downstream of the insert.

MyrEGFP fragment amplified by high fidelity PCR from plasmid pROSA26-mT-mG (www.addgene.org/17787) was InFusion cloned into BamHI site of pGFAP-EGFR* plasmid obtained from Dr. Eric Holland to construct this plasmid. The ~2.2 kb Gfa2 GFAP promoter in the pGFAP-EGFR* plasmid is presumably the same promoter utilized in the GFAP-tva transgenic mouse in Holland EC and Varmus HE https://doi.org/10.1073/pnas.95.3.1218. Addgene NGS sequencing of this plasmid confirmed the Gfa2 promoter sequence in this plasmid is identical to the hGFAP promoter sequences in other Gfa2 plasmids, such as hGFAP-GFP (http://www.addgene.org/40592/) and hGFAP-Cre (http://www.addgene.org/40591/)

DNA (Catalog # 160277-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 160277-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.