SGyA
(Plasmid #160292)

• Purpose: Drives expression of Cas9 and a TdTomato fluorescent marker
• Depositing Lab: Omar Akbari
• Publication: Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi: 10.1038/s41467-021-27333-1.

Backbone

• Vector backbone: attB
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 16663
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Vas promoter
• Alt name: Vasa promoter
• Species: D. melanogaster (fly)
• Insert Size (bp): 2241

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Unknown

Gene/Insert 2

• Gene/Insert name: hSpCas9
• Alt name: Cas9
• Insert Size (bp): 5043
• Tag / Fusion Protein:
  • T2A GFP (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Unknown

Gene/Insert 3

• Gene/Insert name: tdTomato
• Alt name: tdTomato

Cloning Information for Gene/Insert 3

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Unknown

Gene/Insert 4

• Gene/Insert name: CTCF insulator
• Alt name: CTCF insulator
• Insert Size (bp): 308

Cloning Information for Gene/Insert 4

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Unknown

Gene/Insert 5

• Gene/Insert name: Gypsy insulator
• Alt name: Gypsy insulator
• Insert Size (bp): 432

Cloning Information for Gene/Insert 5

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Unknown

Gene/Insert 6

• Gene/Insert name: Left homology arm Y chromosome
• Species: D. melanogaster (fly)
• Insert Size (bp): 811

Cloning Information for Gene/Insert 6

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Unknown

Gene/Insert 7

• Gene/Insert name: Right homology arm Y chromosome
• Insert Size (bp): 813

Cloning Information for Gene/Insert 7

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry