pBAC690-TEF
(Plasmid #160432)

• Purpose: Expresses eGFP under control of the yeast TEF2 promoter
• Depositing Lab: Gary Stormo
• Publication: Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi: 10.1038/s42003-020-01494-z.

Backbone

• Vector backbone: pFA6a–link–yEGFP–Kan
• Backbone size w/o insert (bp): 4894
• Total vector size (bp): 5400
• Vector type: Yeast Expression
• Selectable markers: Kanamycin used with growth in yeast

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: S. cerevisiae TEF2 promoter
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 674
• Promoter: S. cerevisiae TEF2

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ACTTTGTTATGTAGAGTTTTTTTAGCTACC
• 3′ sequencing primer: CATGTTTAGTTAATTATAGTTCGTTGAC

Resource Information

• Supplemental Documents:
  • BAC690_TEF.v2.gb
• A portion of this plasmid was derived from a plasmid made by: Original plasmid was obtained from EUROSCARF. We cloned the TEF2 promoter from yeast genomic DNA.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.05.11.089300v1 for bioRxiv preprint.

Parent vector is described in "Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae", Mark A Sheff , Kurt S ThornYeast 2004 Jun;21(8):661-70. doi: 10.1002/yea.1130. The parent vector was intended for GFP tagging of yeast proteins. We placed the TEF2 promoter directly upstream of GFP for sugar-insensitive expression of GFP under galactose or dextrose conditions.