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(Plasmid #160665)


Item Catalog # Description Quantity Price (USD)
Plasmid 160665 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Structural Genomics Gonsortium
  • Total vector size (bp) 6675
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    Af1521 modified synthetic construct eGFP fusion
  • Alt name
  • Species
    Synthetic; Archaeoglobus fulgidus
  • Insert Size (bp)
  • Promoter LacI
  • Tags / Fusion Proteins
    • hexahistidine_TEV recognition (N terminal on backbone)
    • eGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer T7F
  • 3′ sequencing primer T7R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNIC28-MacroGreen was a gift from Herwig Schüler (Addgene plasmid # 160665 ; ; RRID:Addgene_160665)
  • For your References section:

    MacroGreen, a simple tool for detection of ADP-ribosylated proteins. Garcia-Saura AG, Herzog LK, Dantuma NP, Schuler H. Commun Biol. 2021 Jul 28;4(1):919. doi: 10.1038/s42003-021-02439-w. 10.1038/s42003-021-02439-w PubMed 34321589