saposin B^W
(Plasmid #161759)

• Purpose: Saposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophan
• Depositing Lab: Sam Gellman
• Publication: Kurgan et al Biochemistry. 2021 Apr 13;60(14):1108-1119. doi: 10.1021/acs.biochem.0c00924. Epub 2021 Mar 23.

Backbone

• Vector backbone: pET15b
• Backbone size w/o insert (bp): 5711
• Total vector size (bp): 5948
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: saposin B R38_W
• Alt name: saposin B^W
• Species: H. sapiens (human)
• Insert Size (bp): 237
• Mutation: changed Arginine 38 to Tryptophan
• Promoter: T7
• Tag / Fusion Protein:
  • His-tag (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene Universal Inc.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

R38W amino acid numbering is relative to the partial sequence provided by the deposing laboratory.