GFP-OSH2-2xPH
(Plasmid
#161987)
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PurposeMammalian expression vector, contains two tandem repeats of OSH2 PH domain (PI(4)P biosensor). Fused to eGFP
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Depositing Labs
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Depositing OrganizationNational Institute of Child Health and Human Development (NICHD)
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 161987 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 161987-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 161987-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneeGFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOSH2
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneOSH2 (a.k.a. YDL019C)
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 161987-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 161987-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-OSH2-2xPH was a gift from Tamas Balla & David Bryant (Addgene plasmid # 161987 ; http://n2t.net/addgene:161987 ; RRID:Addgene_161987) -
For your References section:
Maintenance of hormone-sensitive phosphoinositide pools in the plasma membrane requires phosphatidylinositol 4-kinase IIIalpha. Balla A, Kim YJ, Varnai P, Szentpetery Z, Knight Z, Shokat KM, Balla T. Mol Biol Cell. 2008 Feb;19(2):711-21. doi: 10.1091/mbc.e07-07-0713. Epub 2007 Dec 12. 10.1091/mbc.e07-07-0713 PubMed 18077555
