Flag Snail 2SA
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16219||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepCMV-Tag 2B
- Backbone size w/o insert (bp) 4300
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)800
Mutation2SA: S97A, S101A
Entrez GeneSNAI1 (a.k.a. SLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1)
/ Fusion Protein
- Flag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Also see Flag Snail WT author's map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Flag Snail 2SA was a gift from Mien-Chie Hung (Addgene plasmid # 16219 ; http://n2t.net/addgene:16219 ; RRID:Addgene_16219)
For your References section:Dual regulation of Snail by GSK-3beta-mediated phosphorylation in control of epithelial-mesenchymal transition. Zhou BP, Deng J, Xia W, Xu J, Li YM, Gunduz M, Hung MC. Nat Cell Biol. 2004 Oct . 6(10):931-40. 10.1038/ncb1173 PubMed 15448698
Map uploaded by the depositor.