pNG1304
(Plasmid
#162488)
-
PurposeExpresses HelD K239A from Bacillus subtillis
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 162488 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepHis6-HelD
-
Backbone manufacturerhttps://doi.org/10.1093/nar/gku113
-
Modifications to backboneIntroduced a K239A point mutation in helD gene
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameHelD
-
SpeciesBacillus subtillis 168
-
Insert Size (bp)2424
-
MutationChanged Lysine 239 to Alanine (K239A)
-
GenBank IDCAB15350.1
-
Entrez GenehelD (a.k.a. BSU_33450, BSU33450)
-
Tag
/ Fusion Protein
- 6His-V5 (N terminal on backbone)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pNG1304 was a gift from Peter Lewis (Addgene plasmid # 162488 ; http://n2t.net/addgene:162488 ; RRID:Addgene_162488) -
For your References section:
Molecular basis for RNA polymerase-dependent transcription complex recycling by the helicase-like motor protein HelD. Newing TP, Oakley AJ, Miller M, Dawson CJ, Brown SHJ, Bouwer JC, Tolun G, Lewis PJ. Nat Commun. 2020 Dec 18;11(1):6420. doi: 10.1038/s41467-020-20157-5. 10.1038/s41467-020-20157-5 PubMed 33339820
