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pSB-CMV-MCS-Puro cPLA2-mK2-P2A-EGFP-LaminB1
(Plasmid #162566)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 162566 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 11236
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    cytosolic phospholipase a2
  • Alt name
    cPLA2
  • Alt name
    pla2g4aa
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    2962
  • GenBank ID
    30554
  • Entrez Gene
    pla2g4aa (a.k.a. PLA2G4A, cp, cpla2, pla, pla2g4)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mKate2 (C terminal on insert)
    • P2A (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV
  • 3′ sequencing primer BGHR
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Lamin B1
  • Alt name
    LMNB1
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1767
  • GenBank ID
    195816
  • Entrez Gene
    lmnb1 (a.k.a. fc06g01, wu:fc06g01)
  • Promoter SV40
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSB-CMV-MCS-Puro cPLA2-mK2-P2A-EGFP-LaminB1 was a gift from Philipp Niethammer (Addgene plasmid # 162566 ; http://n2t.net/addgene:162566 ; RRID:Addgene_162566)
  • For your References section:

    The Cell Nucleus Serves as a Mechanotransducer of Tissue Damage-Induced Inflammation. Enyedi B, Jelcic M, Niethammer P. Cell. 2016 May 19;165(5):1160-1170. doi: 10.1016/j.cell.2016.04.016. 10.1016/j.cell.2016.04.016 PubMed 27203112