pET28a(+) NRP1 b1b2 (273-586)
(Plasmid
#162733)
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PurposeBacterial expression the b1b2 tandem domains from the rat Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 162733 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepET-28a(+)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHuman NRP1 b1b2 domains (residues 273-586)
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MutationCodon optimised
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Tag
/ Fusion Protein
- 6His
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a(+) NRP1 b1b2 (273-586) was a gift from Brett Collins (Addgene plasmid # 162733 ; http://n2t.net/addgene:162733 ; RRID:Addgene_162733) -
For your References section:
Neuropilin-1 is a host factor for SARS-CoV-2 infection. Daly JL, Simonetti B, Klein K, Chen KE, Williamson MK, Anton-Plagaro C, Shoemark DK, Simon-Gracia L, Bauer M, Hollandi R, Greber UF, Horvath P, Sessions RB, Helenius A, Hiscox JA, Teesalu T, Matthews DA, Davidson AD, Collins BM, Cullen PJ, Yamauchi Y. Science. 2020 Oct 20. pii: science.abd3072. doi: 10.1126/science.abd3072. 10.1126/science.abd3072 PubMed 33082294
Map uploaded by the depositor.
