pET15b/GVcl 1-1066 /*TBM
(Plasmid #162781)

• Purpose: amplification of Gallus gallus Vcl mutated in Tankyrase Binding Domain II
• Depositing Lab: Laura Lafon-Hughes
• Publication: et al PeerJ

Backbone

• Vector backbone: pET15b/GVcl 1-1066 (Addgene #46171)
• Total vector size (bp): 8909
• Modifications to backbone: G454V
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: LB
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Gallus gallus vinculin
• Alt name: VCL
• Species: G. gallus (chicken)
• Insert Size (bp): 3198
• Mutation: changed Gly 454 to Val
• Entrez Gene: VCL (a.k.a. VINC1)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CTGCGACGACATGGGAAAGTAGACTCTCCTGAGGCCCGT
• 3′ sequencing primer: ACGGGCCTCAGGAGAGTCTACTTTCCCATGTCGTCGCAG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We received the plasmid bearing WT GgVcl from addgene, and then mutated it.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry