pMIR-R/Tac1/SG
(Plasmid
#16296)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16296 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMIR-Report
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Backbone manufacturerAmbion
- Backbone size w/o insert (bp) 6500
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Vector typeMammalian Expression, Luciferase
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTac1 3'UTR
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Alt nameTac1
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Alt namePPT-1, PPT-A
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SpeciesH. sapiens (human)
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Insert Size (bp)534
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GenBank IDNM_003182
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Entrez GeneTAC1 (a.k.a. Hs.2563, NK2, NKNA, NPK, TAC2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site SacI (not destroyed)
- 5′ sequencing primer Luc-F
- 3′ sequencing primer M13-F20 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMIR-R/Tac1/SG was a gift from Pranela Rameshwar (Addgene plasmid # 16296 ; http://n2t.net/addgene:16296 ; RRID:Addgene_16296) -
For your References section:
MicroRNAs regulate synthesis of the neurotransmitter substance P in human mesenchymal stem cell-derived neuronal cells. Greco SJ, Rameshwar P. Proc Natl Acad Sci U S A. 2007 Sep 25. 104(39):15484-9. 10.1073/pnas.0703037104 PubMed 17855557