pDESTpK7WG-RPS5Ap-mito
(Plasmid
#163145)
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PurposePlant mitoTALEN vector assembly, Destination vector for plants to express ORFs from entry clone with mitochondrial presequence under the RPS5A promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 163145 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepK7WG2
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Backbone manufacturerVIB, Ghent Univ, Karimi et al. Trends Plant Sci. (2002)
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Vector typePlant Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRPS5A promoter and mitochondrial presequence in front of attR1 in pk7WG
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SpeciesSynthetic
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Gateway destination vector for plants to express ORFs from entry clone with mitochondrial presequence under the RPS5A promoter. Use with Platinum Gate TALEN kit (Kit # 1000000043) and plasmids #158728-#158735.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDESTpK7WG-RPS5Ap-mito was a gift from Shin-ichi Arimura (Addgene plasmid # 163145 ; http://n2t.net/addgene:163145 ; RRID:Addgene_163145) -
For your References section:
Targeted gene disruption of ATP synthases 6-1 and 6-2 in the mitochondrial genome of Arabidopsis thaliana by mitoTALENs. Arimura SI, Ayabe H, Sugaya H, Okuno M, Tamura Y, Tsuruta Y, Watari Y, Yanase S, Yamauchi T, Itoh T, Toyoda A, Takanashi H, Tsutsumi N. Plant J. 2020 Oct 24. doi: 10.1111/tpj.15041. 10.1111/tpj.15041 PubMed 33098708
