|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16332||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript II SK+
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesX. laevis (frog)
Insert Size (bp)2200
Entrez Geneotx1 (a.k.a. otx1-A, xotx1)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Isolated from Harland Stage 30 head library. Expressed in dorsal mesoderm and forebrain like Otx2, but also expressed in germ plasm.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Otx1/pBS was a gift from Peter Klein (Addgene plasmid # 16332 ; http://n2t.net/addgene:16332 ; RRID:Addgene_16332)
Map uploaded by the depositor.