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Addgene

GSK-3beta S9A (Xenopus)/pCS2+
(Plasmid #16333)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16333 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Vertebrate expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GSK-3beta
  • Alt name
    GSK3b
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1200
  • Mutation
    GSK-3 with serine-9 to alanine mutation
  • Entrez Gene
    gsk3b.L (a.k.a. XELAEV_180112401mg, gsk-3, gsk3, gsk3-beta, gsk3b, gsk3beta, xgsk-3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Serine to alanine mutation (s9a) made in Xenopus GSK-3beta originally from Kimelman lab's XG73. For sense transcripts cut with NotI and transcribe with SP6

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GSK-3beta S9A (Xenopus)/pCS2+ was a gift from Peter Klein (Addgene plasmid # 16333 ; http://n2t.net/addgene:16333 ; RRID:Addgene_16333)
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