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Barcode-Set B_2
(Plasmid #163513)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163513 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pGL4.24
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4411
  • Total vector size (bp) 4491
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Parts of DNA sequences from LOC542299 stress-induced protein 1
  • Species
    Zea mays
  • Insert Size (bp)
    80

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CACTGCATTCTAGTTGTGGTT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the Addgene NGS finds a A61T mismatch in the barcode compared to the depositor's supplemental document. The difference is not a concern for plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Barcode-Set B_2 was a gift from Arne S. Schaefer (Addgene plasmid # 163513 ; http://n2t.net/addgene:163513 ; RRID:Addgene_163513)