Barcode-Set C_1
(Plasmid
#163514)
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PurposeEncodes the luciferase reporter gene luc2P+Barcode C1
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Depositing Lab
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Depositing OrganizationCharite Universitaetsmedizin Berlin
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 163514 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepGL4.24
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4411
- Total vector size (bp) 4491
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameParts of DNA sequences from LOC542509 defective kernel 1
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SpeciesZea mays
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Insert Size (bp)80
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer CACTGCATTCTAGTTGTGGTT
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the Addgene NGS finds a A58T and A61T mismatch in the barcode compared to the depositor's supplemental document. The difference is not a concern for plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Barcode-Set C_1 was a gift from Arne S. Schaefer (Addgene plasmid # 163514 ; http://n2t.net/addgene:163514 ; RRID:Addgene_163514)