plRL19
(Plasmid #163634)

• Purpose: L5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).
• Depositing Lab: Jeremy Rock
• Publication: Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00824-2. doi: 10.1016/j.cell.2021.06.033.

Backbone

• Vector backbone: N/A
• Backbone manufacturer: Rock Lab
• Backbone size w/o insert (bp): 3088
• Total vector size (bp): 4204
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Shake at 220rpm. For best yield, grow for >16 hours.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: L5 Integrase
• Alt name: L5 Int
• Species: Mycobacteriophage L5
• Insert Size (bp): 1116
• GenBank ID: M65194.1
• Promoter: Mycobacterial Optimized Promoter (MOP)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • plrl19-addgene-163634.gb
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry