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pAAV_CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40
(Plasmid #163698)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 163698 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV
  • Total vector size (bp) 5608
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40
  • Alt name
    CaMKII-CaMPARI2
  • Species
    Synthetic
  • Insert Size (bp)
    1446
  • Promoter CaMKII fragment
  • Tags / Fusion Proteins
    • FLAG-HA-myc (C terminal on insert)
    • NES_his (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Schreiter lab at Janelia Research Campus

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CaMKII promoter fragment cloned into https://www.addgene.org/101060/ to replace hSyn promoter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV_CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40 was a gift from Gina Turrigiano (Addgene plasmid # 163698 ; http://n2t.net/addgene:163698 ; RRID:Addgene_163698)
  • For your References section:

    Activity labeling in vivo using CaMPARI2 reveals intrinsic and synaptic differences between neurons with high and low firing rate set points. Trojanowski NF, Bottorff J, Turrigiano GG. Neuron. 2021 Feb 17;109(4):663-676.e5. doi: 10.1016/j.neuron.2020.11.027. Epub 2020 Dec 16. 10.1016/j.neuron.2020.11.027 PubMed 33333001