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pCxcl1-Firefly
(Plasmid #163896)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163896 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 163896-D50 50 μg of DNA in Tris buffer $495
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DNA 163896-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGL4.23-GW
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Luciferase
  • Species
    Firefly
  • Promoter Cxcl1

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The mouse Cxcl1 gene promoter was inserted to drive Firefly luciferase expression.

Please visit https://www.biorxiv.org/content/10.1101/2021.12.23.474040v1 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCxcl1-Firefly was a gift from Alexander Ruthenburg (Addgene plasmid # 163896 ; http://n2t.net/addgene:163896 ; RRID:Addgene_163896)

  • For your References section:

    The cardiac lncRNA Chantico directly regulates Cxcl1 chemokine transcription. Hall JM, Kainth AS, Rowton MJ, Lu E, Haddad G, Keplinger AJ, Šćepanović J , Perez-Cervantes C, Chan SSK, Piccirilli JA, Moskowitz IP, Ruthenburg AJ. bioRxiv 2021.12.23.474040 10.1101/2021.12.23.474040
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