PKG I beta WT
(Plasmid #16394)

• Depositing Lab: Bernard Weinstein
• Publication: Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Backbone

• Vector backbone: pHACE
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PKG1 beta
• Alt name: PKG 1
• Species: H. sapiens (human)
• Insert Size (bp): 2060
• Entrez Gene: PRKG1 (a.k.a. AAT8, PKG, PKG1, PRKG1B, PRKGR1B, cGK, cGK 1, cGK1, cGKI, cGKI-BETA, cGKI-alpha)
• Tag / Fusion Protein:
  • HA (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The PKG I beta insert aligns with the listed reference sequence after amino acid 90, contains an EF insertion after the starting methionine and I537V mutation.