PurposeExpresses zebrafish ythdf1 with 3x flag tag, for mRNA injection
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||164488||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeProduction of mRNA for zebrafish injection.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesD. rerio (zebrafish)
Insert Size (bp)1839
Entrez Geneythdf1 (a.k.a. fb49c02, wu:fb49c02, wu:fi19h0, wu:fi19h06, wu:fj87f09, wu:fp06c1, wu:fp06c11, zgc:55657)
- Promoter SP6
/ Fusion Protein
- 3x-flagtag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer ACGCTCAACTTTGGCAGATCCG
- 3′ sequencing primer GGAGCAGATACGAATGGCTACA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSP64T-ythdf1-3xflag was a gift from Antonio Giraldez (Addgene plasmid # 164488 ; http://n2t.net/addgene:164488 ; RRID:Addgene_164488)
For your References section:Ythdf m(6)A Readers Function Redundantly during Zebrafish Development. Kontur C, Jeong M, Cifuentes D, Giraldez AJ. Cell Rep. 2020 Dec 29;33(13):108598. doi: 10.1016/j.celrep.2020.108598. 10.1016/j.celrep.2020.108598 PubMed 33378672