pETM10 with rat Δ192Annexin A11
(Plasmid #164498)

• Purpose: For bacterial expression of rat His-tagged Δ192Annexin A11
• Depositing Lab: Anni Vedeler
• Publication: Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: biom10040660. doi: 10.3390/biom10040660.

Backbone

• Vector backbone: pET
• Backbone manufacturer: Gunter Stier
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: rat Δ192Annexin A11
• Species: R. norvegicus (rat)
• Insert Size (bp): 900
• Mutation: N-terminal truncation
• Entrez Gene: Anxa11
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: Acc65I (not destroyed)
• 5′ sequencing primer: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The cDNAs were obtained by us by using PC12 cells, RT-PCR and specific primers. The cDNAs are inserted in the pETM10 vector between the NcoI and Acc65I (Kpn) sites. The vector, pETM10, is an EMBL vector, which was a generous gift from its maker, Gunter Stier.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry