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StarPort02
(Plasmid #164504)

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  • Purpose
    To generate a stable acceptor cell line with an improved BxbI attP-GA site at the AASV1 locus, between an exogenous human pRPL30 promoter and a tripartite V5/SNAPtag/Blast reporter.
  • Depositing Lab
    Bo Huang
  • Depositing Organization
    University of California, San Francisco (UCSF)
    Located in United States of America
  • Publication
    Huang Lab Starport System Plasmids (unpublished) ( How to cite )
  • Sequence Information

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164504 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    N/A
  • Backbone manufacturer
    Designed by David Brown in the Huang Lab at UCSF
  • Vector type
    Mammalian Expression ; To generate attP-GA acceptor cell lines for BxBI
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    V5-SNAPtag-PDGFR_TM-T2A-Blast
  • Species
    Synthetic
  • Promoter human RPL30

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    StarPort02 was a gift from Bo Huang (Addgene plasmid # 164504 ; http://n2t.net/addgene:164504 ; RRID:Addgene_164504)
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