LjUBQ:MtGLV1:T35s
(Plasmid
#164738)
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PurposeOverexpression of Peptide coding gene (Binary vector)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 164738 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneEC50505
- Backbone size w/o insert (bp) 10475
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Vector typePlant Expression
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Selectable markersNeomycin (select with G418) ; N/A
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMedtr6g043230.1
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Alt nameGLV1
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Alt nameKEH26052.1
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SpeciesMedicago truncatula
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Insert Size (bp)899
- Promoter Lotus japonicus Ubiquitin
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Tag
/ Fusion Protein
- Terminator 35S (C terminal on insert)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloned by Sonali/Shulan
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LjUBQ:MtGLV1:T35s was a gift from Wolf Scheible (Addgene plasmid # 164738 ; http://n2t.net/addgene:164738 ; RRID:Addgene_164738) -
For your References section:
The peptide GOLVEN10 alters root development and noduletaxis in Medicago truncatula. Roy S, Torres-Jerez I, Zhang S, Liu W, Schiessl K, Jain D, Boschiero C, Lee HK, Krom N, Zhao PX, Murray JD, Oldroyd GED, Scheible WR, Udvardi M. Plant J. 2024 May;118(3):607-625. doi: 10.1111/tpj.16626. Epub 2024 Feb 15. 10.1111/tpj.16626 PubMed 38361340
