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pmiCas9-msa
(Plasmid #164833)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164833 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 164833-D50 50 μg of DNA in Tris buffer $495
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DNA 164833-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pJDS246
  • Backbone manufacturer
    Joung lab
  • Backbone size w/o insert (bp) 2466
  • Total vector size (bp) 8268
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miCas9-msa
  • Species
    Synthetic; Streptococcus pyogenes
  • Insert Size (bp)
    5933
  • Mutation
    contains mSA sequence range is 5106-5447 and Brex27 sequence range is 5508-5615.
  • Promoter CMV
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    the original gene was from Addgene#47754

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmiCas9-msa was a gift from Eugene Chen (Addgene plasmid # 164833 ; http://n2t.net/addgene:164833 ; RRID:Addgene_164833)

  • For your References section:

    MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits. Ma L, Ruan J, Song J, Wen L, Yang D, Zhao J, Xia X, Chen YE, Zhang J, Xu J. Nat Commun. 2020 Nov 27;11(1):6082. doi: 10.1038/s41467-020-19842-2. 10.1038/s41467-020-19842-2 PubMed 33247137
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