p273 EF1a_mCherry2_hH11
(Plasmid
#164852)
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PurposemCherry2-expression vector for human H11 locus
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Depositing Lab
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Depositing OrganizationJohns Hopkins School of Public Health
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 164852 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 164852-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 164852-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonePS100010
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Backbone manufacturerOrigene
- Backbone size w/o insert (bp) 2848
- Total vector size (bp) 8315
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry2
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Insert Size (bp)711
- Promoter EF1-alpha
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer none
- 3′ sequencing primer none
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bymCherry2 is subcloned from Addgene #72796 pMK280 (mCherry2-NeoR); EF1-alpha is subcloned from pRAIDRS-P10 R. Brosh et al., Nat. Com. 2016; loxP-Puro-loxP cassette is subcloned from Addgene #22208 TV-hPITX3-Reverse; homology arms for human H11 are amplified from hESCs - H1 (WiCell).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 164852-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 164852-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p273 EF1a_mCherry2_hH11 was a gift from Philip Jordan (Addgene plasmid # 164852 ; http://n2t.net/addgene:164852 ; RRID:Addgene_164852) -
For your References section:
Adaptation of Human Testicular Niche Cells for Pluripotent Stem Cell and Testis Development Research. Pryzhkova MV, Jordan PW. Tissue Eng Regen Med. 2020 Apr;17(2):223-235. doi: 10.1007/s13770-020-00240-0. Epub 2020 Feb 29. 10.1007/s13770-020-00240-0 PubMed 32114677
Map uploaded by the depositor.
