PurposeExpression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||164986||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepX330 (Addgene #42230)
- Backbone size w/o insert (bp) 9703
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Gene/Insert namegRNA against HLA class I
SpeciesH. sapiens (human)
- Cloning method Restriction Enzyme
- 5′ cloning site BbsI (unknown if destroyed)
- 3′ cloning site BbsI (unknown if destroyed)
- 5′ sequencing primer U6-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CRISPR_HLA_class_I_1 was a gift from Matthew Meyerson (Addgene plasmid # 164986 ; http://n2t.net/addgene:164986 ; RRID:Addgene_164986)
For your References section:Antigen identification for HLA class I- and HLA class II-restricted T cell receptors using cytokine-capturing antigen-presenting cells. Lee MN, Meyerson M. Sci Immunol. 2021 Jan 22;6(55). pii: 6/55/eabf4001. doi: 10.1126/sciimmunol.abf4001. 10.1126/sciimmunol.abf4001 PubMed 33483338