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pGEX4T-1-Optimized E6AP
(Plasmid #165100)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 165100 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX4T1
  • Backbone manufacturer
    GE Healthcare
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E6AP isoform 3
  • Alt name
    UBE3A isoform 3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2620
  • Mutation
    codon optimized for E. coli expression
  • Entrez Gene
    UBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A)
  • Tag / Fusion Protein
    • GST from plasmid. (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX4T-1-Optimized E6AP was a gift from Arthur Haas (Addgene plasmid # 165100 ; http://n2t.net/addgene:165100 ; RRID:Addgene_165100)
  • For your References section:

    E6AP/UBE3A ubiquitin ligase harbors two E2~ubiquitin binding sites. Ronchi VP, Klein JM, Haas AL. J Biol Chem. 2013 Apr 12;288(15):10349-60. doi: 10.1074/jbc.M113.458059. Epub 2013 Feb 25. 10.1074/jbc.M113.458059 PubMed 23439649