pGEX4T1-ISG15 C78SN13Y
(Plasmid #165106)

• Purpose: bacterial expressio of a GST fusion of Human ISG15 C78SN13Y
• Depositing Lab: Arthur Haas
• Publication: Narasimhan et al J Biol Chem. 2005 Jul 22. 280(29):27356-65.

Backbone

• Vector backbone: pGEX4T-1
• Backbone manufacturer: GE Healthcare
• Backbone size w/o insert (bp): 4900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ISG15
• Alt name: UCRP
• Species: H. sapiens (human)
• Insert Size (bp): 471
• Mutation: C78SN13Y; The sequence of ISG15-C78S/N13Y contains a cysteine to serine mutation at residue 78 which has been found to increase stability of the protein during purification, concentration, and storage. It also contains an asparagine to tyrosine mutation at residue 13 to produce a solvent accessible site for labeling with 125Iodine
• Entrez Gene: ISG15 (a.k.a. G1P2, IFI15, IMD38, IP17, UCRP, hUCRP)
• Tag / Fusion Protein:
  • GST from plasmid. (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry