Purposemammalian expression and localization
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||165130||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameNsp13 K289A
Speciescodon optimized SARS-COV-2
Insert Size (bp)1800
Entrez GeneORF1ab (a.k.a. GU280_gp01)
- Promoter CMV
/ Fusion Protein
- EGFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer GTCGTAACAACTCCGCCC
- 3′ sequencing primer CGTCCAGCTCGACCAGGATG (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byInserts were prepared by PCR with templates that have been published elsewhere: Gordon et al., 2020a.
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Nsp13 K289A-EGFP was a gift from Bruno Antonny (Addgene plasmid # 165130 ; http://n2t.net/addgene:165130 ; RRID:Addgene_165130)
For your References section:A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in different cell types. Miserey-Lenkei S, Trajkovic K, D'Ambrosio JM, Patel AJ, Copic A, Mathur P, Schauer K, Goud B, Albanese V, Gautier R, Subra M, Kovacs D, Barelli H, Antonny B. Biol Cell. 2021 Jul;113(7):311-328. doi: 10.1111/boc.202000158. Epub 2021 May 10. 10.1111/boc.202000158 PubMed 33666950